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| Registros recuperados : 1 | |
| 1. |  | SOUZA, H. A. de; MELO, M. D.; PRIMO, A. A.; VIEIRA, L. V.; POMPEU, R. C. F. F.; GUEDES, F. L.; NATALE, W. Use of organic compost containing waste from small ruminants in corn production. Revista Brasileira de Ciência do Solo, v. 40, p. 1-16, e0150385, 2016.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Meio-Norte. |
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| Registros recuperados : 1 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
15/07/2002 |
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Data da última atualização: |
08/11/2018 |
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Autoria: |
MENDES, B. M. J.; BOSCARIOL, R. L.; MOURÃO FILHO, F. de A. A.; ALMEIDA, W. A. B. de. |
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Afiliação: |
BEATRIZ MADALENA JANUZZI MENDES, Universidade de São Paulo - USP/Centro de Energia Nuclear na Agricultura - CENA; RAQUEL LUCIANA BOSCARIOL, Universidade de São Paulo - USP/Centro de Energia Nuclear na Agricultura - CENA; WELITON ANTONIO BASTOS DE ALMEIDA, Escola Superior de Agricultura Luiz de Queiroz - Esalq/Departamento de Produção Vegetal. |
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Título: |
Agrobacterium-mediated genetic transformation of 'Hamlin' sweet orange. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 7, p. 955-961, jul. 2002 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Transformação genética de laranja "Hamlin" via agrobacterium. |
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Conteúdo: |
The development and optimization of efficient transformation protocols is essential in new citrus breeding programs, not only for rootstock, but also for scion improvement. Transgenic 'Hamlin' sweet orange (Citrus sinensis (L.) Osbeck) plants were obtained by Agrobacterium tumefaciens-mediated transformation of epicotyl segments collected from seedlings germinated in vitro. Factors influencing genetic transformation efficiency were evaluated including seedling incubation conditions, time of inoculation with Agrobacterium and co-culture conditions. Epicotyl segments were adequate explants for transformation, regenerating plants by direct organogenesis. Higher percentage of transformation was obtained with explants collected from seedlings germinated in darkness, transferred to 16 hours photoperiod for 2-3 weeks, and inoculated with Agrobacterium for 15-45 min. The best co-culture condition was the incubation of the explants in darkness, for three days in culture medium supplemented with 100 mM of acetosyringone. Genetic transformation was confirmed by performing ß-glucoronidase (GUS) assays and, subsequently, by PCR amplification for the nptII and GUS genes. |
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Palavras-Chave: |
epicótilo; métodos de melhoramento; plântulas; transgênicos; transgenics. |
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Thesagro: |
Citrus Sinensis. |
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Thesaurus NAL: |
breeding methods; epicotyls; seedlings. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/22582/1/pab1158.pdf
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Marc: |
LEADER 02045naa a2200277 a 4500
001 1107474
005 2018-11-08
008 2002 bl uuuu u00u1 u #d
100 1 $aMENDES, B. M. J.
245 $aAgrobacterium-mediated genetic transformation of 'Hamlin' sweet orange.
260 $c2002
500 $aTítulo em português: Transformação genética de laranja "Hamlin" via agrobacterium.
520 $aThe development and optimization of efficient transformation protocols is essential in new citrus breeding programs, not only for rootstock, but also for scion improvement. Transgenic 'Hamlin' sweet orange (Citrus sinensis (L.) Osbeck) plants were obtained by Agrobacterium tumefaciens-mediated transformation of epicotyl segments collected from seedlings germinated in vitro. Factors influencing genetic transformation efficiency were evaluated including seedling incubation conditions, time of inoculation with Agrobacterium and co-culture conditions. Epicotyl segments were adequate explants for transformation, regenerating plants by direct organogenesis. Higher percentage of transformation was obtained with explants collected from seedlings germinated in darkness, transferred to 16 hours photoperiod for 2-3 weeks, and inoculated with Agrobacterium for 15-45 min. The best co-culture condition was the incubation of the explants in darkness, for three days in culture medium supplemented with 100 mM of acetosyringone. Genetic transformation was confirmed by performing ß-glucoronidase (GUS) assays and, subsequently, by PCR amplification for the nptII and GUS genes.
650 $abreeding methods
650 $aepicotyls
650 $aseedlings
650 $aCitrus Sinensis
653 $aepicótilo
653 $amétodos de melhoramento
653 $aplântulas
653 $atransgênicos
653 $atransgenics
700 1 $aBOSCARIOL, R. L.
700 1 $aMOURÃO FILHO, F. de A. A.
700 1 $aALMEIDA, W. A. B. de
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 37, n. 7, p. 955-961, jul. 2002
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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